Post-Traumatic Stress Response and Appendicitis in Children—Clinical Usefulness of Selected Biomarkers

Acute appendicitis is an inflammatory process which one of the most frequent global causes surgical interventions in children. The goal study was to determine whether acute phase proteins, that is, C-reactive protein (CRP), procalcitonin (PCT) and neutrophil gelatinase-associated lipocalin (NGAL), interleukin 6 (IL-6), transforming growth factor-beta1 (TGF-β1) cortisol (HC) play a role pathomechanism post-trauma stress response organism establish impact applied procedure and/or inflammation on their concentrations. An additional purpose clinical usefulness studied biomarkers diagnostics appendicitis. CRP concentrations were quantified via immunoturbidimetric method, while levels IL-6 PCT assessed using bead-based multiplexed immunoassay system microplate format (Luminex xMAP technology); NGAL, TGF-β1 determined enzyme-linked immunosorbent assay (ELISA) technique. All investigated assayed twice, i.e., immediately before surgery 12–24 h after its completion. Significant increases CRP, found all children subjected laparoscopic surgeries (p = 0.001, p 0.006, 0.009, respectively) open (classic) 0.016, 0.044, compared initial patients undergoing classical moreover presented with significant 0.002, 0.022, NGAL procedures. In group surgery, induced increase concentration, whereas this biomarker caused by type performed procedure. Simultaneously assaying 0.008, 0.000, may prove useful practice, enabling diagnosis paediatric reporting hospital abdominal pains, addition data from anamnesis or ultrasound examination. confirms participation examined post-injury reaction trauma.